作者
Rosie E Bradshaw, Yanan Guo, Andre D Sim, M Shahjahan Kabir, Pranav Chettri, Ibrahim K Ozturk, Lukas Hunziker, Rebecca J Ganley, Murray P Cox
发表日期
2016/2
期刊
Molecular Plant Pathology
卷号
17
期号
2
页码范围
210-224
简介
We present genome‐wide gene expression patterns as a time series through the infection cycle of the fungal pine needle blight pathogen, Dothistroma septosporum, as it invades its gymnosperm host, Pinus radiata. We determined the molecular changes at three stages of the disease cycle: epiphytic/biotrophic (early), initial necrosis (mid) and mature sporulating lesion (late). Over 1.7 billion combined plant and fungal reads were sequenced to obtain 3.2 million fungal‐specific reads, which comprised as little as 0.1% of the sample reads early in infection. This enriched dataset shows that the initial biotrophic stage is characterized by the up‐regulation of genes encoding fungal cell wall‐modifying enzymes and signalling proteins. Later necrotrophic stages show the up‐regulation of genes for secondary metabolism, putative effectors, oxidoreductases, transporters and starch degradation. This in‐depth through‐time …
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