作者
Constance M John, Mingfeng Liu, Nancy J Phillips, Zhijie Yang, Courtney R Funk, Lindsey I Zimmerman, J McLeod Griffiss, Daniel C Stein, Gary A Jarvis
发表日期
2012/11
期刊
Infection and immunity
卷号
80
期号
11
页码范围
4014-4026
出版商
American Society for Microbiology
简介
The interaction of the immune system with Neisseria commensals remains poorly understood. We have previously shown that phosphoethanolamine on the lipid A portion of lipooligosaccharide (LOS) plays an important role in Toll-like receptor 4 (TLR4) signaling. For pathogenic Neisseria, phosphoethanolamine is added to lipid A by the phosphoethanolamine transferase specific for lipid A, which is encoded by lptA. Here, we report that Southern hybridizations and bioinformatics analyses of genomic sequences from all eight commensal Neisseria species confirmed that lptA was absent in 15 of 17 strains examined but was present in N. lactamica. Mass spectrometry of lipid A and intact LOS revealed the lack of both pyrophosphorylation and phosphoethanolaminylation in lipid A of commensal species lacking lptA. Inflammatory signaling in human THP-1 monocytic cells was much greater with pathogenic than with …
学术搜索中的文章
Lack of lipid A pyrophosphorylation and functional lptA reduces inflammation by Neisseria commensals
CM John, M Liu, NJ Phillips, Z Yang, CR Funk… - Infection and immunity, 2012