作者
Philip Supply, Michael Marceau, Sophie Mangenot, David Roche, Carine Rouanet, Varun Khanna, Laleh Majlessi, Alexis Criscuolo, Julien Tap, Alexandre Pawlik, Laurence Fiette, Mickael Orgeur, Michel Fabre, Cécile Parmentier, Wafa Frigui, Roxane Simeone, Eva C Boritsch, Anne-Sophie Debrie, Eve Willery, Danielle Walker, Michael A Quail, Laurence Ma, Christiane Bouchier, Grégory Salvignol, Fadel Sayes, Alessandro Cascioferro, Torsten Seemann, Valérie Barbe, Camille Locht, Maria-Cristina Gutierrez, Claude Leclerc, Stephen D Bentley, Timothy P Stinear, Sylvain Brisse, Claudine Médigue, Julian Parkhill, Stéphane Cruveiller, Roland Brosch
发表日期
2013/2
期刊
Nature genetics
卷号
45
期号
2
页码范围
172-179
出版商
Nature Publishing Group US
简介
Global spread and limited genetic variation are hallmarks of M. tuberculosis, the agent of human tuberculosis. In contrast, Mycobacterium canettii and related tubercle bacilli that also cause human tuberculosis and exhibit unusual smooth colony morphology are restricted to East Africa. Here, we sequenced and analyzed the whole genomes of five representative strains of smooth tubercle bacilli (STB) using Sanger (4–5× coverage), 454/Roche (13–18× coverage) and/or Illumina DNA sequencing (45–105× coverage). We show that STB isolates are highly recombinogenic and evolutionarily early branching, with larger genome sizes, higher rates of genetic variation, fewer molecular scars and distinct CRISPR-Cas systems relative to M. tuberculosis. Despite the differences, all tuberculosis-causing mycobacteria share a highly conserved core genome. Mouse infection experiments showed that STB strains are less …
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P Supply, M Marceau, S Mangenot, D Roche… - Nature genetics, 2013