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In the present study, the edible plant Zingiber officinalis ethanol and chloroform extracts were prepared and their cytotoxic effects versus human cervical cancer (HeLa) and mouse fibroblast (L929) cell-lines were investigated. HeLa and L929 cell lines in 96 well microplates were cultivated for 24 h with initial concentrations of 8× 04 cells/ml and 7.4× 104 cells/ml, respectively. After that the cultures were treated with different dilutions of the extracts and incubated for 48 h. The growth inhibition was determined to the 50% inhibition concentration (IC50). IC50 values were reported as±95% confidence intervals (±95% CI) by using Graph Pad Prism (San Diego, CA). The antibacterial activity of the ginger extracts was tested by the paper disc diffusion technique. The results of the morphological observation and MTT test indicated that the cytotoxic activity of the extracts were dose dependent. IC50 values versus L929 and HeLa cells were found to be 87.28 µg/ml and 74.32 µg/ml, respectively, for the chloroform extract, while the ethanol extract showed IC50 values at 101 µg/ml and 33.78 µg/ml, respectively. Moreover, the extracts were evaluated for their antimicrobial activities against Klebsiella pneumoniae, Salmonella thyphimurium, Bacillus cereus, Enterococcus fecalis and Staphylococcus aureus. The antimicrobial activity results showed that the ginger extracts inhibited the growth of five out of eight microorganisms but had no effect on the growth of Escherichia coli ATCC11230, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus epidermidis ATCC 12228.