作者
R Blawid, JMF Silva, T Nagata
发表日期
2017/5
来源
Annals of Applied Biology
卷号
170
期号
3
页码范围
301-314
出版商
Blackwell Publishing Ltd
简介
Small‐scale sequencing has improved substantially in recent decades, culminating in the development of next‐generation sequencing (NGS) technologies. Modern NGS methods have helped the discovery of many new plant viruses. Nevertheless, there is still a need to establish solid assembly pipelines targeting small genomes characterised by low identities to known viral sequences. Here, we describe and discuss the fundamental steps required for discovering and sequencing new plant viral genomes by NGS. A practical pipeline and standard alternative tools used in NGS analysis are presented.
引用总数
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