作者
Roberto Russo, Davide Monaco, Marcello Rubessa, Khalid A El-Bahrawy, Ashraf El-Sayed, Nicola A Martino, Benedicte Beneult, Francesca Ciannarella, Maria E Dell’Aquila, Giovanni M Lacalandra, Manuel Filioli Uranio
发表日期
2014/12
期刊
Reproductive Biology and Endocrinology
卷号
12
页码范围
1-10
出版商
BioMed Central
简介
Background
Reproductive biotechnologies in dromedary camel (Camelus dromedarius) are less developed than in other livestock species. The in vitro maturation (IVM) technology is a fundamental step for in vitro embryo production (IVP), and its optimization could represent a way to increase the success rate of IVP. The aim of the present study was to investigate the bioenergy/oxidative status of dromedary camel oocytes before and after IVM by confocal microscopy 3D imaging.
Methods
Oocytes were retrieved by slicing ovaries collected at local slaughterhouses. Recovered oocytes were examined before and after IVM culture for nuclear chromatin configuration and bioenergy/oxidative status, expressed as mitochondria (mt) distribution and activity, intracellular Reactive Oxygen Species (ROS) levels and distribution and mt/ROS colocalization …
学术搜索中的文章
R Russo, D Monaco, M Rubessa, KA El-Bahrawy… - Reproductive Biology and Endocrinology, 2014
