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Mycotoxins are significant contaminants in human foodstuffs and feeds, and have demonstrated serious effects in both humans and animal’s health. They are produced as secondary metabolites by different genera of fungi. Enniatins are Fusarium mycotoxins which belong to the category of ‘emerging’mycotoxins, mainly contaminated cereals. Last decade has greatly increased the research on this class of mycotoxins due to their constantly increasing presence in the entire food chain. The main analytical technique that used for the analysis and identification of Enniatins is the liquid chromatograph-mass spectrometry. Enniatins have biological activities, that indicate the potent toxicity of these toxic metabolites. The European Commission has not set maximum permitted levels of Enniatins in foods and feeds.