作者
LF Marques-Santos, G Grassi, E Bergami, C Faleri, T Balbi, A Salis, G Damonte, L Canesi, I Corsi
发表日期
2018/9/14
期刊
Nanotoxicology
卷号
12
期号
8
页码范围
847-867
出版商
Taylor & Francis
简介
In order to assess the impact of nanoplastics on marine species, polystyrene nanoparticles (PS NPs) have been largely used as model particles. Here we studied the effects of 50 nm amino-modified PS-NH2 on Mediterranean sea urchin Paracentrotus lividus immune system cells (coelomocytes) in the presence of celomic fluid (CF) and at different NP concentrations (1, 5, 10, and 25 μg mL−1) and experimental conditions (absence or presence of EDTA). PS-NH2 acquired a protein corona once incubated with CF, dominated by the toposome precursor protein (TPP). In short-term cultures, a significant concentration- and time-dependent decrease in lysosomal membrane stability and apoptotic-like nuclear alterations were observed in phagocytes upon exposure to PS-NH2 (10 and 25 µg mL−1) in CF but they resulted abolished in the presence of EDTA confirming the role of TPP in triggering PS-NH2 …
学术搜索中的文章
LF Marques-Santos, G Grassi, E Bergami, C Faleri… - Nanotoxicology, 2018