发明者
Richard Owczarzy, Bernardo Moreira, Yong You, Mark Aaron Behlke, Joseph Alan Walder
发表日期
2011/11/8
专利局
US
专利号
8055451
专利申请号
11970509
简介
The invention relates to methods and systems for predicting orestimating the melting temperature of duplex nucleic acids, in the presence of divalent cations, particularly duplexes of oligonucleotides which may be used as, for example, but not limited to primers or probes in PCR and/or hybridization assays. The methods and algorithms use novel formulas, hav ing terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of divalent cation salt conditions on the melting temperature.
学术搜索中的文章
R Owczarzy, B Moreira, Y You, MA Behlke, JA Walder - US Patent 8,055,451, 2011
