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(Design-Expert, 2005) using these concentrations and 2% w/v of chitosan, with the objective to 151 find one or more optimal mixtures with a broad spectrum of action, effective simultaneously 152 against yeast, gram-positive and gram-negative bacteria. The above MIC values were selected, 153 because the other MIC were either too high, as in the case of P. aeruginosa, showing values of MIC 154 greater than 7500 ppm for carvacrol and values of MIC of 16000 ppm for GSE, or too low, such as 155