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Antimicrobial activity of Amazonian oils Andiroba and Copaiba against Paenibacillus larvae has been recently determined, indicating their potential use for the control of American Foulbrood Disease (AFB), but the use of essential oils in the environment still represents a challenge. The oils present several volatile elements in its composition, such high volatility being the cause of a sharp decline in antimicrobial activity. In this context the nanostructuration of these amazon oils may decrease the volatile characteristic of such products. The following research aimed to evaluate the activity of nanoemulsions prepared with Andiroba and Copaiba oils against Paenibacillus species. The toxicity of nanoemulsions has also been investigated with larvae and adult worker bees. Nanoemulsions (NE1, 10% Andiroba oil; NE2, 10% Copaiba oil; and NE3, 10% medium-chain triglyceride as negative control) were prepared in a …