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The large genus Centaurea consists of more than 700 species [1], from which 75 species are found in Iran. They are widespread around the country. In Iran their common name is Gole-gandom. Centaurea behen (Gole-Gandome-Talaei in Persian) is a perennial plant 60–200 cm tall that is traditionally used as a tonic for jaundice, cystic fibrosis, and impotence. It is an Irano–Turanian plant grows on stony hillsides, meadows, and along the roadsides [2]. Several sesquiterpene lactones of the guaianolide type (cynaripicrin, arguerin B, deacylcynaropicrine, grosshemin, and 4β, 15-dihydro-3-dehydrosolstitialin A)[3, 4], two methoxylated flavone (crisimaritin and jeceosidin)[4, 5], and a flavonoid aglycone (luteolin)[6] were isolated from the leaves of Centaurea behen. In this study, fractionation of the petroleum ether and chloroform extracts of Centaurea behen L. resulted in the isolation of two methoxylated flavones, two …