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Identifying diseases and evaluating tissue function and viability can be performed by subjective or objective methods. However, subjective techniques may be inaccurate and non-optical objective techniques may be relatively expensive and time-consuming. Then, these techniques may not be suitable for clinical applications that require immediate assessment and intervention. Fluorescence spectroscopy (FS) is one of the optical techniques with great potential for medical diagnostics and surgical guidance. This potential is associated to the possibility of label-free techniques biochemical sensitivity without contrast agents. For clinical applications, fluorescence can be used to assess biomolecular content of respiratory metabolism involving NAD(P)H and FAD. In addition, changes in collagen, elastin, porphyrin, pyridoxine, and tryptophan content can potentially be detected. One way to collect epifluorescence signals …