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This study focused on the comparative analysis of biosorption performance of Delonix regia seed pod toward the removal of Rhodamine B (RB) from simulated solution using native (DRSP) and chemically treated form (ADRSP). The surface morphology, structural analysis, textural properties, and thermal analysis of DRSP and ADRSP were examined using scanning electron microscopy (SEM), BET analysis, Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA), respectively. FTIR analysis concluded that surface functional groups like hydroxyl –OH stretching, C–N stretching, and C = C stretching of the aromatic ring were largely responsible for the attachment of RB. The chemical treatment enhanced the surface morphology of D. regia seed in terms of heterogeneity, distinct depth cavities, and irregular pores responsible for RB biosorption. The biosorption of RB was investigated …