作者
Taejoon Won, Megan K Wood, David M Hughes, Monica V Talor, Zexu Ma, Jowaly Schneider, John T Skinner, Beejan Asady, Erin Goerlich, Marc K Halushka, Allison G Hays, Deok-Ho Kim, Chirag R Parikh, Avi Z Rosenberg, Isabelle Coppens, Roger A Johns, Nisha A Gilotra, Jody E Hooper, Andrew Pekosz, Daniela Čiháková
发表日期
2022/1/1
期刊
EBioMedicine
卷号
75
出版商
Elsevier
简介
Background
Thromboembolism is a life-threatening manifestation of coronavirus disease 2019 (COVID-19). We investigated a dysfunctional phenotype of vascular endothelial cells in the lungs during COVID-19.
Methods
We obtained the lung specimens from the patients who died of COVID-19. The phenotype of endothelial cells and immune cells was examined by flow cytometry and immunohistochemistry (IHC) analysis. We tested the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the endothelium using IHC and electron microscopy.
Findings
The autopsy lungs of COVID-19 patients exhibited severe coagulation abnormalities, immune cell infiltration, and platelet activation. Pulmonary endothelial cells of COVID-19 patients showed increased expression of procoagulant von Willebrand factor (VWF) and decreased expression of anticoagulants thrombomodulin and endothelial protein …
学术搜索中的文章
T Won, MK Wood, DM Hughes, MV Talor, Z Ma… - EBioMedicine, 2022