作者
Riqiang Yan, Stephen Small, Claude Desplan, Charles R Dearolf, James E Darnell
发表日期
1996/2/9
期刊
Cell
卷号
84
期号
3
页码范围
421-430
出版商
Elsevier
简介
A Drosophila Stat gene (D-Stat) with a zygotic segmental expression pattern was identified. This protein becomes phosphorylated on Tyr-704 when coexpressed in Schneider cells with a Drosophila janus kinase (JAK), Hopscotch (HOP). The phosphorylated protein binds specifically to the consensus sequence TTCCCGGAA. Suppressor mutations of hopTum-l, a dominant hyperactive allele of hop whose phenotype is hematocyte overproduction and tumor formation, were selected. One of these mutantsstatHJ, mapped to the same chromosomal region (92E) as does D-Stat, had an incompletely penetrant pair rule phenotype, and exhibited aberrant expression of the pair rule gene even skipped (eve) at the cellular blastoderm stage. Two D-STAT-binding sites were identified within the eve stripe 3 enhancer region. Mutations in either of the STAT-binding sites greatly decreased the stripe 3 expression in transgenic …
引用总数
学术搜索中的文章
R Yan, S Small, C Desplan, CR Dearolf, JE Darnell - Cell, 1996