作者
Bobo Dang, Marco Mravic, Hailin Hu, Nathan Schmidt, Bruk Mensa, William F DeGrado
发表日期
2019/4
期刊
Nature methods
卷号
16
期号
4
页码范围
319-322
出版商
Nature Publishing Group US
简介
Site-specific protein cleavage is essential for many protein-production protocols and typically requires proteases. We report the development of a chemical protein-cleavage method that is achieved through the use of a sequence-specific nickel-assisted cleavage (SNAC)-tag. We demonstrate that the SNAC-tag can be inserted before both water-soluble and membrane proteins to achieve fusion protein cleavage under biocompatible conditions with efficiency comparable to that of enzymes, and that the method works even when enzymatic cleavages fail.
学术搜索中的文章
B Dang, M Mravic, H Hu, N Schmidt, B Mensa… - Nature methods, 2019