作者
JoAnn McGee, Richard J Goodyear, D Randy McMillan, Eric A Stauffer, Jeffrey R Holt, Kirsten G Locke, David G Birch, P Kevin Legan, Perrin C White, Edward J Walsh, Guy P Richardson
发表日期
2006/6/14
期刊
Journal of Neuroscience
卷号
26
期号
24
页码范围
6543-6553
出版商
Society for Neuroscience
简介
Sensory hair bundles in the inner ear are composed of stereocilia that can be interconnected by a variety of different link types, including tip links, horizontal top connectors, shaft connectors, and ankle links. The ankle link antigen is an epitope specifically associated with ankle links and the calycal processes of photoreceptors in chicks. Mass spectrometry and immunoblotting were used to identify this antigen as the avian ortholog of the very large G-protein-coupled receptor VLGR1, the product of the Usher syndrome USH2C (Mass1) locus. Like ankle links, Vlgr1 is expressed transiently around the base of developing hair bundles in mice. Ankle links fail to form in the cochleae of mice carrying a targeted mutation in Vlgr1 (Vlgr1/del7TM), and the bundles become disorganized just after birth. FM1-43 [N-(3-triethylammonium)propyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide] dye loading and whole-cell recordings …
引用总数
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JA McGee, RJ Goodyear, DR McMillan, EA Stauffer… - Journal of Neuroscience, 2006