作者
Ji‐Yi Zhang, Yi‐Ching Lee, Ivone Torres‐Jerez, Mingyi Wang, Yanbin Yin, Wen‐Chi Chou, Ji He, Hui Shen, Avinash C Srivastava, Christa Pennacchio, Erika Lindquist, Jane Grimwood, Jeremy Schmutz, Ying Xu, Manoj Sharma, Rita Sharma, Laura E Bartley, Pamela C Ronald, Malay C Saha, Richard A Dixon, Yuhong Tang, Michael K Udvardi
发表日期
2013/4
期刊
The Plant Journal
卷号
74
期号
1
页码范围
160-173
简介
Switchgrass (Panicum virgatum L.) is a perennial C4 grass with the potential to become a major bioenergy crop. To help realize this potential, a set of RNA‐based resources were developed. Expressed sequence tags (ESTs) were generated from two tetraploid switchgrass genotypes, Alamo AP13 and Summer VS16. Over 11.5 million high‐quality ESTs were generated with 454 sequencing technology, and an additional 169 079 Sanger sequences were obtained from the 5′ and 3′ ends of 93 312 clones from normalized, full‐length‐enriched cDNA libraries. AP13 and VS16 ESTs were assembled into 77 854 and 30 524 unique transcripts (unitranscripts), respectively, using the Newbler and pave programs. Published Sanger‐ESTs (544 225) from Alamo, Kanlow, and 15 other cultivars were integrated with the AP13 and VS16 assemblies to create a universal switchgrass gene index (PviUT1.2) with 128 058 …
学术搜索中的文章
JY Zhang, YC Lee, I Torres‐Jerez, M Wang, Y Yin… - The Plant Journal, 2013