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Geographical origin and authenticity of food are topics of interest for both consumers and producers. Among the different indicators used for traceability studies, ⁸⁷Sr/⁸⁶Sr isotopic ratio has provided excellent results. In this study, two analytical approaches for wine sample pre-treatment, microwave and low temperature mineralisation, were investigated to develop accurate and precise analytical method for ⁸⁷Sr/⁸⁶Sr determination. The two procedures led to comparable results (paired t-test, with t < t_crit). Furthermore, the precision of the whole analytical procedure was evaluated by using a control sample (wine sample), processed during each sample batch (calculated Relative Standard Deviation, RSD%, equal to 0.002%. Lambrusco PDO (Protected Designation of Origin) wines coming from four different vintages (2009, 2010, 2011 and 2012) were pre-treated according to the best procedure and their isotopic …