作者
Maciej Wiznerowicz, Didier Trono
发表日期
2003/8/15
期刊
Journal of virology
卷号
77
期号
16
页码范围
8957-8961
出版商
American Society for Microbiology
简介
RNA interference has emerged as a powerful technique to downregulate the expression of specific genes in cells and in animals, thus opening new perspectives in fields ranging from developmental genetics to molecular therapeutics. Here, we describe a method that significantly expands the potential of RNA interference by permitting the conditional suppression of genes in mammalian cells. Within a lentivirus vector background, we subjected the polymerase III promoter-dependent production of small interfering RNAs to doxycycline-controllable transcriptional repression. The resulting system can achieve the highly efficient and completely drug-inducible knockdown of cellular genes. As lentivirus vectors can stably transduce a wide variety of targets both in vitro and in vivo and can be used to generate transgenic animals, the present system should have broad applications.
引用总数
20042005200620072008200920102011201220132014201520162017201820192020202120222023202437536672757679675757504244292324231914114