作者
Üzeyir Dogan, Esin Kasap, Demet Cetin, Zekiye Suludere, Ismail Hakkı Boyaci, Canan Türkyılmaz, Nusret Ertas, Ugur Tamer
发表日期
2016/10/5
期刊
Sensors and Actuators B: Chemical
卷号
233
页码范围
369-378
出版商
Elsevier
简介
In this study, a fast and sensitive sandwich assay has been developed with the combination of immunomagnetic separation (IMS) and fluorescence techniques to enumerate Escherichia coli (E.coli). Iron oxide core gold shell (Fe3O4@Au) magnetic nanoparticles were prepared and modified with biotinylated antibodies specific to E.coli. Fluorescence labels have been constructed by preparing chitosan coated CdTe quantum dots (CdTe QDs) with a diameter of 3 ± 1 nm. The amounts of magnetic nanoparticles and CdTe QDs are optimized to get the best sensitivity. The calibration graph fluorescence intensity versus E.coli concentration was linear in the range of 102–108 cfu mL−1 with a coefficient of determination (R2) of 0.9905. The limit of detection (LOD) and limit of quantification (LOQ) values are calculated as 30 and 100 cfu mL−1, respectively. Selectivity of the method is examined by applying the same …
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Ü Dogan, E Kasap, D Cetin, Z Suludere, IH Boyaci… - Sensors and Actuators B: Chemical, 2016