作者
Andrea Bosso, L Pirone, Rosa Gaglione, Katia Pane, A Del Gatto, L Zaccaro, S Di Gaetano, D Diana, R Fattorusso, Elisa Pedone, Valeria Cafaro, HP Haagsman, A Van Dijk, MR Scheenstra, Anna Zanfardino, Orlando Crescenzi, Angela Arciello, Mario Varcamonti, EJA Veldhuizen, Alberto Di Donato, Eugenio Notomista, Eliodoro Pizzo
发表日期
2017/9/1
期刊
Biochimica et Biophysica Acta (BBA)-General Subjects
卷号
1861
期号
9
页码范围
2342-2353
出版商
Elsevier
简介
Background
Host defence peptides (HDPs) are evolutionarily conserved components of innate immunity. Human HDPs, produced by a variety of immune cells of hematopoietic and epithelial origin, are generally grouped into two families: beta structured defensins and variably-structured cathelicidins. We report the characterization of a very promising cryptic human HDP, here called GVF27, identified in 11-hydroxysteroid dehydrogenase-1 β-like protein.
Methods
Conformational analysis of GVF27 and its propensity to bind endotoxins were performed by NMR, Circular Dichroism, Fluorescence and Dynamic Light Scattering experiments. Crystal violet and WST-1 assays, ATP leakage measurement and colony counting procedures were used to investigate antimicrobial, anti-biofilm, cytotoxicity and hemolytic activities. Anti-inflammatory properties were evaluated by ELISA.
Results
GVF27 possesses significant …
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A Bosso, L Pirone, R Gaglione, K Pane, A Del Gatto… - Biochimica et Biophysica Acta (BBA)-General Subjects, 2017