作者
Eliza de Oliveira Cardoso, Bruno José Conti, Karina Basso Santiago, Fernanda Lopes Conte, Lucas Pires Garcia Oliveira, Rodrigo Tavanelli Hernandes, Marjorie de Assis Golim, José Maurício Sforcin
发表日期
2017/1
期刊
Journal of Pharmacy and Pharmacology
卷号
69
期号
1
页码范围
99-108
出版商
Oxford University Press
简介
Objectives
Propolis is a natural product with a complex chemical composition. Its isolated compounds exert biological activities; however, its synergistic effects are unknown. The involvement of phenolic acids (caffeic – Caf, dihydrocinnamic – Cin and p-coumaric – Cou) alone or in combination was investigated in the action of propolis in human monocytes.
Methods
Cell viability was analysed by MTT assay; TNF-α, IL-6 and IL-10 production by enzyme-linked immunosorbent assay (ELISA); cell markers expression by flow cytometry; colony-forming units were counted to assess the microbicidal activity; and H2O2 production was analysed by colorimetric assay.
Key findings
Treatments did not affect monocytes viability. Propolis and combinations containing Caf enhanced TNF-α production by resting cells. Propolis, Cin, Cou and Caf + Cin stimulated IL-6 …
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EO Cardoso, BJ Conti, KB Santiago, FL Conte… - Journal of Pharmacy and Pharmacology, 2017