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This study aimed to investigate the protective effect of diets supplemented with different combinations of three antioxidants (nano‑selenium (NanoSe), vitamin C and E) on rainbow trout (Oncorhynchus mykiss) under sub-lethal concentration of ammonia exposure. For this goal, triplicate groups of 25 fish/tank (11.89 ± 0.34 g) were subjected to ammonia stress (0.024 mg/L) and fed with three experimental diets as T₁ (0.1 mg/kg NanoSe, 100 mg/kg vitamin C and 30 mg/kg vitamin E), T₂ (0.2 mg/kg NanoSe, 200 mg vitamin C and 60 mg/kg vitamin E), T₃ (0.3 mg/kg NanoSe, 300 mg/kg vitamin C and 90 mg/kg vitamin E) and a basal diet as control, for 40 days. The obtained results showed that the fish fed with T₂ and T₃ diets had significantly higher final weight, weight gain, specific growth rate, protein efficiency ratio and lipid efficiency ratio, when compared to T₁ and the control group. Also, all the supplemented diet …