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OBJECTIVE(S): To examine the effect of exogenous exposure to hydrogen peroxide (H₂O₂) and tumor necrosis factor (TNF)-α on mouse metaphase II (MII) oocyte spindle structure and to examine the potential benefits of supplementing the culture media with vitamin C. DESIGN: Prospective study. SETTING: Research laboratory in a tertiary hospital. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Microtubule changes and alterations in chromosomal alignment. RESULT(S): Both concentration- and time-dependent alterations were seen in spindle structure after exposure to H₂O₂. An H₂O₂ concentration as low as 12.5 μM increased the odds of an oocyte with altered microtubule and chromosome alignment (score ≥3) by 93%. Significantly increased damage was seen with increasing period of incubation. Higher scores were seen after exposure to both TNF-α alone and in combination with H₂O₂ …