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A bioflocculant is a kind of biodegradable polymeric flocculants produced by many microorganisms including bacteria, fungi and actinomycetes during their growth. Bioflocculant have special advantages such as safety for ecosystems, potential flocculating effects, biodegradability and harmlessness to humans and the environment. Based on these unique properties it may potentially be applied in production industries for various purposes. In this study, a total of thirty nine (39) bacteria were isolated from three different wastewater samples from Oyo and Osun states. The isolates were screened for bioflocculant production using kaolin solution (5g/L) as a test material. There was a significant different (P≥ 0.05) in the flocculating ability of the isolates. The percentage flocculating activity ranged from 03.00p–75.63a% in which isolate TB3 had the highest followed in order by isolates TM3, AM6 and API, while isolate AP3 had the least. Six isolates with the best flocculating activity selected and identified phenotypically were: Enterobacter asburiae AM6, Streptococcus plurextorum AP1,