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Electromagnetic frequencies (EF) generated from different laboratory devices and appliances can interact with biological systems, including humans. Several studies have assessed the effect of electromagnetic frequencies on the growth of microorganisms, which revealed that EF could inhibit bacterial growth, decrease colony-forming units, or decrease viability depending on the field strength and frequency. Accordingly, in this study, we aimed to assess the effect of the antibiotics extracted electromagnetic spectrums on the growth of Staphylococcus aureus (S. aureus). Drugs digital electromagnetic spectrums of a mixture of antibiotics were generated using a DigiConPro® VITATEC device emitted using the supplied antenna and applied into a glass tube containing nutrient broth inoculated with S. aureus, and optical density was measured hourly for 6 hours. The post-exposure effect of antibiotics' electromagnetic spectrums was also determined on bacteria subcultured into blood agar. Antibiotics’ electromagnetic spectrums