作者
Richard D Patten, David DeNofrio, Mohamad El-Zaru, Rahul Kakkar, Jessica Saunders, Flore Celestin, Kenneth Warner, Hassan Rastegar, Kamal R Khabbaz, James E Udelson, Marvin A Konstam, Richard H Karas
发表日期
2005/5/3
期刊
Journal of the american college of cardiology
卷号
45
期号
9
页码范围
1419-1424
出版商
American College of Cardiology Foundation
简介
Objectives
We examined the effect of mechanical unloading with ventricular assist device (VAD) therapy on myocardial inducible nitric oxide synthase (iNOS) expression and cardiomyocyte apoptosis in patients with end-stage heart failure (HF).
Background
Despite advances in medical therapy, HF continues to be a progressive and ultimately fatal disorder. High levels of iNOS expression are present in the myocardium of failing hearts, suggesting a potential role for iNOS in HF progression.
Methods
Inducible NOS protein expression was analyzed by Western blotting and cardiomyocyte apoptosis by terminal deoxynucleotidyltransferase dUTP nick end-labeling (TUNEL) in myocardial samples from failing hearts. Included in these analyses were tissues from 9 patients at the time of transplantation (HF-transplant group), 10 patients at the time of VAD insertion (pre-VAD group), and 11 patients undergoing transplant after …
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RD Patten, D DeNofrio, M El-Zaru, R Kakkar… - Journal of the american college of cardiology, 2005