作者
Sachiko Kanki, Vincent FM Segers, Weitao Wu, Rahul Kakkar, Joseph Gannon, Stanislas U Sys, Anthony Sandrasagra, Richard T Lee
发表日期
2011/7
期刊
Circulation: Heart Failure
卷号
4
期号
4
页码范围
509-518
出版商
Lippincott Williams & Wilkins
简介
Background
Stromal cell-derived factor-1 (SDF-1) is a chemoattractant of stem/progenitor cells, and several studies have shown that SDF-1 may improve ventricular function after infarction. SDF-1 is cleaved by proteases including matrix metalloproteinase-2 (MMP-2) and CD26/dipeptidylpeptidase-4 (DPP-4), which are activated in injured tissues.
Methods and Results
We investigated the biodistribution and functional roles of SDF-1 in experimental ischemia/reperfusion injury in rats. Radiolabeled SDF-1 given by intracoronary injection was selectively concentrated in ischemic myocardium. The enhanced uptake of SDF-1 in ischemic myocardium was not mediated by its receptor, CXCR4. Mass spectrometry and Western analyses showed that SDF-1 was cleaved by DPP-4 in plasma and myocardium, whereas a bioengineered MMP-2/DPP-4–resistant form of SDF-1, SSDF-1(S4V), was highly stable. A single dose of …
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S Kanki, VFM Segers, W Wu, R Kakkar, J Gannon… - Circulation: Heart Failure, 2011