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The protective effects of the aqueous extract of Argemone mexicana (Linn.) whole plant, against Aniline induced spleen failure in male albino rats was investigated. The animal was divide in to four groups. Group I kept control. Group II was treated by Aniline hydrochloride (2 mmol/kg) intoxicated for 7 days. Group III was treated by AMEE (Argemone mexicana Ethanolic extract)(250 mg/kg po) treated for 7 days & AH, Group IV: AMEE (500 mg/kg po) treated for 7 days & AH, Group V: Livfit (150 mg/kg po) for 7 days. The blood was collected (5 ml) by cardiac puncture. Spleen weight, Relative spleen weight and morphological characteristics of spleen was recorded along with hematological parameters such as hemoglobin, RBC, Total WBC, platelet count, reticulocyte conc., serum albumin and methemoglobin conc. were determined in blood samples.