作者
AD Martins, MG Alves, RL Bernardino, TR Dias, BM Silva, PF Oliveira
发表日期
2014/9
期刊
European journal of nutrition
卷号
53
页码范围
1383-1391
出版商
Springer Berlin Heidelberg
简介
Purpose
Many health benefits have been attributed to tea (Camellia sinensis (L.)), and tea infusions are used as dietary agent and included in food supplements. Herein, we report the effect of a white tea (WTEA) extract in Sertoli cell (SC) metabolism. The SC is responsible for the nutritional support of the developing germ cells.
Methods
An aqueous WTEA extract was prepared and analyzed by 1H-NMR. Rat SCs were cultured with or without the WTEA extract. mRNA and protein levels of glucose transporters (GLUT1 and GLUT3), phosphofructokinase, lactate dehydrogenase (LDH) and monocarboxylate transporter 4 were determined by qPCR and western blot. LDH activity was assessed and metabolite production/consumption determined by 1H-NMR.
Results
WTEA-exposed SCs presented decreased protein and …
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