作者
Marco Trizzino, Paolo Audisio, Gloria Antonini, Alessio De Biase, Emiliano Mancini
发表日期
2009/5/1
期刊
Molecular Phylogenetics and Evolution
卷号
51
期号
2
页码范围
215-226
出版商
Academic Press
简介
A comparative analysis of ITS2 sequences and secondary structures in 89 species of pollen beetles of the subfamily Meligethinae (Coleoptera, Nitidulidae) was performed. The ITS2 folding pattern was highly conserved and comparable with the general model proposed for eukaryotes. Simple sequence repeats (SSRs) were responsible for most of the observed nucleotide variability (∼1–3%) and length variation (359–459bp). When plotted on secondary structures, SSRs mapped in expansion segments positioned at the apices of three ITS2 helices (‘A’, ‘B’ and ‘D1’) and appeared to have evolved under mechanisms of compensatory slippage. Homologies among SSRs nucleotides could not be unambiguously assigned, and thus were not useful to resolve phylogeny. However, slippage-derived motifs provided some preliminary genetic support for newly proposed taxonomic arrangements of several genera and …
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M Trizzino, P Audisio, G Antonini, A De Biase… - Molecular Phylogenetics and Evolution, 2009