作者
Wai-Heng Lua, Wei-Li Ling, Chinh Tran-To Su, Joshua Yi Yeo, Chandra Shekhar Verma, Birgit Eisenhaber, Frank Eisenhaber, Samuel Ken-En Gan
发表日期
2017/3/4
期刊
Cell Cycle
卷号
16
期号
5
页码范围
457-467
出版商
Taylor & Francis
简介
The IgA receptor, Fcar (CD89) consists of 5 sequence segments: 2 segments (S1, S2) forming the potential signal peptide, 2 extracellular EC domains that include the IgA binding site, and the transmembrane and cytoplasmic tail (TM/C) region. Numerous Fcar splice variants have been reported with various combinations of the sequence segments mentioned above. Here, we report a novel splice variant termed variant APD isolated from a healthy volunteer that lacks only the IgA-binding EC1 domain. Despite possessing the complete signal peptide S1+S2, the variant APD is only found in the intracellular space whereas the wild-type variant 1 is efficiently secreted and variant 4 leaks to the extracellular space. Further mutational experiments involving signal peptide replacements, cleavage site modifications, and studies on alternative isoforms demonstrate that despite the completeness of the signal peptide motif, the …
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WH Lua, WL Ling, CTT Su, JY Yeo, CS Verma… - Cell Cycle, 2017