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Cyclodextrin glucanotransferase (CGTase) was produced when the Bacillus sp. TS1-1 was grown in a medium containing sago starch, yeast extract, phosphorus and mineral salt sources, using shake flask mode at 37°C for 24h. Response surface methodology (RSM) was applied to optimize the medium constituents with respect to CGTase production and activity. A 2⁴ full factorial design (first order model) was carried out to identify the significant effect of medium components towards CGTase production. The variables involved in this initial screening study were sago starch, yeast extract, K₂HPO₄ and MgSO₄·7H₂O. Statistical analysis of results have shown that only sago starch and yeast extract have a significant effect on CGTase production. A second-order model was proposed by using 2² central composite design to represent the production CGTase activity as a function of sago starch and yeast extract. The …