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Biodiesels from various biodegradable feedstocks are being researched to replace conventional fuels. This study aims to analyse and compare the combustion behaviour of a CI engine with different generations of biodiesel by evaluating its performance in terms of heat release, temperature, pressure, fuel consumption, thermal efficiency, and ignition delay. The research was performed on a direct injection, one-cylinder, liquid-cooled CI engine using diesel and a 20 % blend level of biodiesel made from three different generations of biodiesel, i.e., palm oil, Jatropha oil, and microalgae oil. The performance of the diesel engine was evaluated with a variable load of 25 to 100 %, and a comparison was made between the various fuels. The findings show that the value lies between 31.52 and 34.80 J/deg. for heat release rate, 70.11–73.11 bars for cylinder pressure, 12.71–13.94 deg. for ignition delay, 551.97–568.13 K …