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Horseradish peroxidase (HRP) was used to determine the location in the spinal cord of neurons projecting to the superior cervical ganglion of the rat. HRP was applied to the proximal cut end of the cervical sympathetic trunk, close to its entry into the superior cervical ganglion. After survival times of 3, 6, or 9 days, the animals were sacrificed and their spinal cords were processed to visualize HRP using diaminobenzidine, benzidine dihydrochloride, or tetramethylbenzidine. Labeled neurons were found only ipsilateral to the site of HRP application and were restricted to spinal segments C8‐T5. Ninety percent of these neurons were located in segments T1‐T3. Similar numbers of labeled neurons were found at survival times of 3 and 6 days and the mean number ± S.E.M. for 11 experiments at these two survival times was 1575 ± 89. Nine days after application of HRP the mean number of labeled cells and the density …