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HIV-1-infected individuals progressively loss CD4⁺ T cells leading to immunosuppression and raising the risk of opportunistic infections. CD8⁺ T-cells play an important role in the immune response against virus infections through their TCR.

To evaluate the CD8-TCR repertoire and immunologic markers in HIV-1-infected patients.

Ten chronic HIV-1-infected individuals on prolonged effective antiretroviral treatment (ART) were analyzed at baseline (before treatment interruption), after at least one year of treatment interruption (TI) and after at least one year from ART resume (TR). Twenty-four TCR-Vβ gene families were analyzed by a modified CDR3 spectratyping method in isolated CD8⁺ T-cells. Immune activation, exhaustion and subpopulation markers were analyzed by flow cytometry.

Expansion of Vβ10, Vβ14 and Vβ15 families, associated with low cell activation and …