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Nonwoven mats of electrospun fibers have great potential for use in antibacterial activity, especially wound dressing. Our goal is to fabricate electrospun biopolymer nanofibers from polyvinyl alcohol (PVA)/Centella asiatica (CA) as potential wound dressings using the electrospinning technique. CA extracts were loaded into the biopolymer nanofibers with different contents (0, 1, 3, 5 and 7 %w/v). Field emission scanning electron microscopy (FE-SEM) was used to examine the morphology of the biopolymer nanofibers. FE-SEM micrographs revealed the biopolymer fibers to be round and smooth, with average diameters ranging from 100 to 450 nm. The mechanical properties of the biopolymer nanofibers decreased as the concentration of CA extract increased, and the antibacterial activity assays of the biopolymer nanofibers with the addition of CA extract were inhibition zone of 7 –15 mm for all bacteria, indicating …