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Elasticity assessment based on bulk wave velocity (BWV) measurements is the most popular technique to characterize the anisotropic stiffness tensor in cortical bone. Typically, a cuboid bone specimen is cut with its sides along the different anatomical directions. Then, the velocity of shear and longitudinal waves propagating along different directions are assessed, from which stiffness coefficients are calculated. Despite the importance of obtaining accurate elasticity values for bone research, there is no generally accepted protocol to measure BWV and the precision of the technique has been seldom investigated. The purpose of this work is to critically assess the method to measure BWV on cuboid specimens in terms of ultrasound frequency, specimen size and signal processing technique. In this study, we measured polycarbonate specimens of different dimensions and 55 human bone specimens with different …