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Amlodipine besylate is a potent calcium channel blocker used for the treatment of hypertension, congestive heart failure and angina pectoris. Amlodipine besylate avoids the adverse effect of amlodipine in racemic mixtures. A highly precise and cost effective RP-HPLC method with retention time of 2.60 minutes was developed for the estimation of amlodipine besylate in tablet dosage form, by fixing the parameters as WATERS C18 column 250 mm× 4.6 mm (5μm), with mobile phase as acetonitrile: 70mM potassium dihydrogen orthophosphate buffer: methanol (15: 30: 55) and pH adjusted to 3.0 using OPA. Mobile phase flow rate was maintained at 1.0 ml/min and detected at 240nm.