作者
Claire Galand, Juan Manuel Leyva-Castillo, Juhan Yoon, Alex Han, Margaret S Lee, Andrew NJ McKenzie, Michael Stassen, Michiko K Oyoshi, Fred D Finkelman, Raif S Geha
发表日期
2016/11/1
期刊
Journal of Allergy and Clinical Immunology
卷号
138
期号
5
页码范围
1356-1366
出版商
Mosby
简介
Background
Cutaneous exposure to food allergens predisposes to food allergy, which is commonly associated with atopic dermatitis (AD). Levels of the epithelial cytokine IL-33 are increased in skin lesions and serum of patients with AD. Mast cells (MCs) play a critical role in food-induced anaphylaxis and express the IL-33 receptor ST2. The role of IL-33 in patients with MC-dependent food anaphylaxis is unknown.
Objective
We sought to determine the role and mechanism of action of IL-33 in patients with food-induced anaphylaxis in a model of IgE-dependent food anaphylaxis elicited by oral challenge of epicutaneously sensitized mice.
Methods
Wild-type, ST2-deficient, and MC-deficient KitW-sh/W-sh mice were epicutaneously sensitized with ovalbumin (OVA) and then challenged orally with OVA. Body temperature was measured by means of telemetry, Il33 mRNA by means of quantitative PCR, and IL-33, OVA …
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C Galand, JM Leyva-Castillo, J Yoon, A Han, MS Lee… - Journal of Allergy and Clinical Immunology, 2016