作者
Juan Manuel Leyva-Castillo, Claire Galand, Shunya Mashiko, Robert Bissonnette, Alex McGurk, Steven F Ziegler, Chen Dong, Andrew NJ McKenzie, Marika Sarfati, Raif S Geha
发表日期
2020/6/1
期刊
Journal of Allergy and Clinical Immunology
卷号
145
期号
6
页码范围
1606-1614. e4
出版商
Mosby
简介
Background
Atopic dermatitis skin lesions demonstrate increased expression of IL-25 by keratinocytes and increased numbers of type 2 innate lymphoid cells (ILC2s) that express high levels of IL-25 receptor (IL-25R). IL-13 is expressed in atopic dermatitis skin lesions and plays an important role in pathogenesis of the disease.
Objective
Our aim was to determine the role of IL-25 and ILC2s in a mouse model of antigen-driven allergic skin inflammation.
Methods
Wild-type mice; mice that express an Il13-driven enhanced green fluorescent protein; and mice that lack IL-25R, IL-25 in keratinocytes, or IL-13 or IL-25R in ILC2s were subjected to acute or chronic epicutaneous sensitization with ovalbumin. Sensitized skin was examined by histology for epidermal thickening. Cellular infiltrates were analyzed for surface markers and intracellular expression of enhanced green fluorescent protein by flow cytometry. Gene …
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JM Leyva-Castillo, C Galand, S Mashiko, R Bissonnette… - Journal of Allergy and Clinical Immunology, 2020