查看文章

Skin colonization with Staphylococcus aureus aggravates atopic dermatitis and exaggerates allergic skin inflammation in mice. IL-4 receptor α (IL-4Rα) blockade is beneficial in atopic dermatitis and reduces S aureus skin colonization through unknown mechanisms. The cytokine IL-17A restrains S aureus growth.

This study sought to examine the effect of IL-4Rα blockade on S aureus colonization at sites of allergic skin inflammation in mice and determine the mechanism involved.

BALB/c mice were epicutaneously sensitized with ovalbumin (OVA). Immediately after, PSVue 794-labeled S aureus strain SF8300 or saline was applied and a single dose of anti-IL-4Rα blocking antibody, a mixture of anti-IL-4Rα and anti-IL-17A blocking antibodies, or IgG isotype controls were administered intradermally. S aureus load was assessed 2 days later by in vivo imaging and enumeration of colony …