查看文章

The most cultivated freshwater species is rainbow trout in aquaculture worldwide. This study was aimed at pharmacokinetics of florfenicol administrated to rainbow trout (Oncorhyncus mykiss) by oral gavages (through a tube leading down the throat to the stomach) and medicated feed. 132 healthy rainbow trout weighing 140±10g were randomly selected and after 2 weeks acclimation period, florfenicol were administrated as oral gavages and medicated feed at single dose 10 mg/kg-1 body weight (BW) to individual fish. Plasma samples were collected at 0, 0.5, 1, 3, 6, 9, 12, 24, 36, 48 and 72 h after feeding and analyzed by high performance liquid chromatography (HPLC) method. The data obtained from plasma concentrations of florfenicol after oral gavages and feed medicated routes were analyzed by SPSS version 16, Mann-Whitney U, ANOVA tests and (P< 0.05) was considered significant. The maximum concentration (Cmax) was gained at 12 h for gavages route (4.68 µg/ml-1), but at 9 h for medicated feed (6.1 µg/ml-1). Themaximum level concentration of florfenicol in medicated feed route was higher than oral gavages route (P< 0.05). It seems that feed can increase absorbance of florfenicol. Also, interestingly the Cmax in medicated feed route rapidly reached and the decreasing process of drug showed less elimination in versus of time.