作者
David Azoulay, Hector I Cohen, Eugene Dementiev, Elizabeth Eshel, Luiza Akria, Ety Shaoul, Netanel Horowitz
发表日期
2020/9
期刊
Cytometry Part B: Clinical Cytometry
卷号
98
期号
5
页码范围
449-453
出版商
John Wiley & Sons, Inc.
简介
Background
Differential diagnosis between diffuse large B‐cell lymphoma (DLBCL) and follicular lymphoma (FL) becomes a challenge when adequate biopsy is unavailable. The present study aimed to investigate the applicability of DNA cell cycle analysis by flow cytometry (FC) for differentiating between CD10+ DLBCL and FL.
Methods
Data were collected from 57 specimens where CD5−/CD10+/light chain restricted B cells were detected. DNA staining was performed using the Coulter DNA Prep Kit. Cell cycle fractions were evaluated by automatic analysis using the ModFit LT software.
Results
Histopathological analysis confirmed the diagnosis of CD10+ FL in 30 specimens (52.6%), CD10+ DLBCL in 24 specimens (42.1%), and CD10+ Burkitt lymphoma in 3 specimens (5.3%). A significantly higher rate of DNA aneuploidy was detected among CD10+ DLBCL than FL specimens (50 vs. 13.3% respectively, p …
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D Azoulay, HI Cohen, E Dementiev, E Eshel, L Akria… - Cytometry Part B: Clinical Cytometry, 2020