作者
Bo Wang, Michal Herman-Edelstein, Philip Koh, Wendy Burns, Karin Jandeleit-Dahm, Anna Watson, Moin Saleem, Gregory J Goodall, Stephen M Twigg, Mark E Cooper, Phillip Kantharidis
发表日期
2010/7/1
期刊
Diabetes
卷号
59
期号
7
页码范围
1794-1802
出版商
American Diabetes Association
简介
OBJECTIVE
Increased deposition of extracellular matrix (ECM) within the kidney is driven by profibrotic mediators including transforming growth factor-β (TGF-β) and connective tissue growth factor (CTGF). We investigated whether some of their effects may be mediated through changes in expression of certain microRNAs (miRNAs).
RESEARCH DESIGN AND METHODS
Proximal tubular cells, primary rat mesangial cells, and human podocytes were analyzed for changes in the expression of key genes, ECM proteins, and miRNA after exposure to TGF-β (1–10 ng/μl). Tubular cells were also infected with CTGF-adenovirus. Kidneys from diabetic apoE mice were also analyzed for changes in gene expression and miRNA levels.
RESULTS
TGF-β treatment was associated with morphologic and phenotypic changes typical of epithelial-mesenchymal transition (EMT …
引用总数
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