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Sir, Creatinine is one of the most common analyte used as the indicator of glomerular filtration rate (GFR) and kidney function. In 1886, Jaffe reported a reaction of creatinine and picric acid in an alkaline medium forming complex having absorbance maxima at 520 nm. The reaction has been brought to use for the measurement of creatinine by Folin and Wu in 1919. Since then, Jaffe’s reaction has been used for estimation of creatinine in serum and urine sample. For more than 90 years, this method is in use for the measurement of creatinine concentration due to its simplicity and low cost. Creatinine is produced as the byproduct of creatine metabolism and is transported through bloodstream to the kidneys for excretion. Malfunctioning of kidney is reflected by lowering of the amount of creatinine in urine and rise of its level in blood. Creatinine levels in blood and urine also indicate the creatinine clearance, accounting …