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The chemokine receptor XCR1 is known to be selectively expressed by cross-presenting dendritic cells (DCs), while its ligand XCL1/lymphotactin is mainly produced by activated CD8⁺ T cells and natural killer cells. Recent studies have shown that XCL1-antigen fusion proteins efficiently induce CD8⁺ T cell responses by preferentially delivering antigens to XCR1⁺ DCs. However, XCL1 per se was found to be a poor adjuvant for induction of CD8⁺ T cell responses. XCL1 is unique because of its lack of one of the two disulfide bonds commonly conserved in all other chemokines and thus has an unstable structure with a relatively weak chemokine activity. In the present study, we generated a variant form of murine XCL1 termed mXCL1-V21C/A59C that contained a second disulfide bond to stabilize its chemokine structure. We confirmed that mXCL1-V21C/A59C had much more potent chemotactic and calcium mobilization activities than the wild type XCL1 (mXCL1-WT). Intradermal injection of mXCL1-V21C/A59C, but not that of mXCL1-WT, significantly increased the accumulation of XCR1⁺CD103⁺ DCs in the injection site, and most of the accumulated XCR1⁺CD103⁺ DCs were found to take up co-injected ovalbumin (OVA). Furthermore, recruited XCR1⁺CD103⁺ DCs efficiently migrated to the draining lymph nodes and stayed for a prolonged period of time. Consequently, mXCL1-V21C/A59C strongly induced OVA-specific CD8⁺ T cells. The combination of OVA and mXCL1-V21C/A59C well protected mice from E.G7-OVA tumor growth in both prophylactic and therapeutic protocols. Finally, memory CTL responses were efficiently induced in mice …