作者
NM Denihan, BH Walsh, SN Reinke, BD Sykes, R Mandal, DS Wishart, DI Broadhurst, GB Boylan, DM Murray
发表日期
2015/5/1
期刊
Clinical Biochemistry
卷号
48
期号
7-8
页码范围
534-537
出版商
Elsevier
简介
Objectives
Metabolomics is defined as the comprehensive study of all low molecular weight biochemicals, (metabolites) present in an organism. Using a systems biology approach, metabolomics in umbilical cord blood (UCB) may offer insight into many perinatal disease processes by uniquely detecting rapid biochemical pathway alterations. In vitro haemolysis is a common technical problem affecting UCB sampling in the delivery room, and can hamper metabolomic analysis. The extent of metabolomic alteration which occurs in haemolysed samples is unknown.
Design and methods
Visual haemolysis was designated by the laboratory technician using a standardised haemolysis index colour chart. The metabolomic profile of haemolysed and non-haemolysed UCB serum samples from 69 healthy term infants was compared using both 1H-NMR and targeted DI and LC–MS/MS approach.
Results
We identified 43 …
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